GrainGenes Sequence Report: BQ162361
Sequence
BQ162361
Contig
NSFT03P2_Contig4995
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ162361
NCBI UniGene
Ta.23852
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001050212.1 Os03g0374400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6BL 6DL
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0761_H10_P19ZY Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0761_H10_P19, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0761_H10_P19
Probe
WHE0761_H10_P19
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gccaacaacaaggccaaaatacatgttggggtgattaaccacatgaaact
aatcaagtgctccctccgttcacttttataagtcatttcagacaactgaa
atcgagttgttttgcacgtctacacgccttataacagtgaacagagggag
tagtaaaaccatacatatttatctagttagacaggtgcatccacctgctg
tcagagcctagctacctatttttctctggtgaattgaatgaatacagaca
tcatacgacatgtaaaagctacaggatatgcacgtcctgatggccatttt
ttagtgcagtcaccccagcagagccagcagcaggttggtggtcctctgag
atatcatctgggccttttatgttgtttagcgacttcttggcgtaaactgt
gaaggcggccgtgagggcagcggcagcgacgaacgagattatgctgtaga
ctatctccactgtagtcaacttatgcttgccatacttcacatcggccaac
gtacgtatcaaccgcccgctatagatatagatgaatgcctcaggtgtcat
tccggcaactgaaccacatacataagggctgaatttgatttctgtcacag
ccacggcatagtta
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ162361
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||