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GrainGenes Sequence Report: BQ162458

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Sequence	BQ162458
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC359616
wEST map position	BQ162458
NCBI UniGene	Ta.44727
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3AS 5BL 5DL 7DS
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0418_h05_o10zT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0418_h05_o10, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0418_h05_o10
Probe	WHE0418_h05_o10
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	taaccgagcatatcctctgaaacttaaaatgggaccaaatatattccgta atcaagacttctggacggatgctaggtatgtattagtttggtttggtttc atctcaaagattcagcgcaacagaccctgctgttacctcatgaataagct gaatatttctgctgctagcgagtaacagcaatgagaaggtagaaaattaa

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