GrainGenes Sequence Report: BQ162573
Sequence
BQ162573
Contig
Ta.8891.1.S1_at NSFT03P2_Contig3113
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ162573
NCBI UniGene
Ta.40257
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001051974.1 Os03g0860600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS 4BS
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0434_B02_C04ZT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0434_B02_C04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0434_B02_C04
Probe
WHE0434_B02_C04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttggggggcctcaatcaactcattttatttt
atttttttgagactcttccatagtagagctacatatgtaccccgtactga
aactgcatgcatgcatgctctgttcttcagtcgatggacttaattttgag
caactgacactacatgcatcgcatgcattctggctacagtagttttgatg
ggatgtactcagagcctcaggtgctgcagcgcagagctaccgtcgaggcc
cttggccctgttgtgccggaccagctccgccattgtcgtgctcctgtacc
gcgcctcgccgtcggcgatgattggcgctagcactctcgtggacgtggcc
acgtcctgccttcggaagaacaggccacagagacgcgaggcccgatgctc
tgggacaccacacggtgct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ162573
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||