GrainGenes Sequence Report: BQ162585
Sequence
BQ162585
Contig
Ta.23959.1.S1_at Ta.23959.2.S1_a_at NSFT03P2_Contig13858
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC335188
wEST map position
BQ162585
NCBI UniGene
Ta.56227
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001047458.1 Os02g0621100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL 7AS 7DS
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0435_F09_L17ZT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0435_F09_L17, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0435_F09_L17
Probe
WHE0435_F09_L17
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttgatgaagaataagctcgggcagtgaagcaa
gttaactgcaattgctaggtaaactccggacgcacacacagtgacagact
gacaacacacatactaattaagtcccacttgctctgcatcaggcattttt
agttggttttactacatccaaaccccaaaatatgactggctgacaccgcc
ccttgcatctatct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ162585
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||