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GrainGenes Sequence Report: BQ162589

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Sequence	BQ162589
Contig	TaAffx.11860.1.S1_s_at NSFT03P2_Contig3115
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC333128
wEST map position	BQ162589
NCBI UniGene	Ta.48820
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001136466.1 hypothetical protein LOC100216577 [Zea mays]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6BL
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0436_E06_J12ZT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0436_E06_J12, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0436_E06_J12
Probe	WHE0436_E06_J12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttaccccaaatccatatccacatcttattgaggaatgtacaactttt cttttgagaaatatacaacatatccatgaggccatagaccgatatacata catgtacatgtgtggtaaatacactgaaaacttggtcagttgcgggcagt taatactccctccgtccggaaatacttgtcctagaaatgattgtatctag acttattttagttatagatctaggacaagtatttccggacgggggagaac atatcaaatggtatttcccctgctgcccgcgacaacgaaatttccttcat ggtcaacctccataattaccaatccatccttatgcccgatcataaccctt ctcaagtcttccgtaatactacatcgcaatgcatgaacttcatagaggct gaagttaagtatcccctcgagcctgtcattacttttggaagcataggatg ggaacagacggtactgactctacttgtgtgcccttcaagtgttttaatac aactatttgtctgcaaatcc

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