GrainGenes Sequence Report: BQ165944

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Sequence

BQ165944

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ165944

NCBI UniGene

Ta.32903

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001064700.1 Os10g0442800 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

1BL

1DL

Clone Library

Wheat vernalized crown cDNA library

Tissue

Crown tissue of seedling

Developmental Stage

Five-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0815_E08_J15ZT Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0815_E08_J15, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0815_E08_J15

Probe

WHE0815_E08_J15

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttttttttttgacctcacaaacccaggcatagcc
attatttagtgatgatagcattaggcttgccgcttgccaaatctcctgtt
tttagttatcatgttctctgaattgtatggtaaacagcaactcaagtggc
atttcagccgttgctgtttttgctaatatctctggatgtcaaagtcataa
agaggaacatctcatccatattttgagtcattgtgttctcacaagcttgg
gtgtccacttgtgccatgtaagccatctaccatctccagctttaatacca
gcgatcctcctcttcagatcaaggcctttcctttttttctcatcactagc
caattcttttgaatggcttatcaggggatgctctggtgttccaaggtcac
tgctatcgacaatcttctgcaccatctccagcacctcgctcatcttaggt
ctataccttgcatggcgcaccaggcacttgtttgccagcgaggcaatcct
gactgccgatttcatgctgtag


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