GrainGenes Sequence Report: Ta.10583.1.A1

GrainGenes Sequence Report: Ta.10583.1.A1_at

Sequence

BQ166045

Contig

Ta.10583.1.A1_at

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001043066.1 Os01g0372700 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat vernalized crown cDNA library

Tissue

Crown tissue of seedling

Developmental Stage

Five-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0806_D12_G24ZT Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0806_D12_G24, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0806_D12_G24

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttttttttaccaaaaagataattttttatgctat
aaaaaagagtcacaaacatatatgcacacgtattactcctagtctcagtt
atggacaacttagtttacacttactatgctttacacctactaccactata
ccctagctagttatcagactatggacaacttgtgctattgcttcagaggc
caatcagatcttcataggtcggccttcccagggtatctcgggaaggggat
gacatctctgatgttgtcgaggccagtggcaaagaggagcatcctctcta
ggcctagcccgaacccgctgtgcttcacggatccatagcgtcgaaggtcc
agatagaactcataggattccataggcaaacctgcgtccagtatccgttg
tttaaggatatcgaagcgctcctccctctgacttccaccaatcaattcac
caaccttgggaactagaacatccatggcagccacggtcttctggtcatcg
ttga


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