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GrainGenes Sequence Report: NSFT03P2_Contig13307

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Sequence
BQ166287
Contig
Ta.14122.1.S1_at
NSFT03P2_Contig13307
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC316855
wEST map position
BQ166287
NCBI UniGene
Ta.14122
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_190071.1 protein kinase-related [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AL
5BL
5DL
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0838_H03_O06ZT Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0838_H03_O06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0838_H03_O06
Probe
WHE0838_H03_O06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttatttttttttttttctttaacaattcaaaaaatttgc
agattctgatagtacaaaaaaatatgtccgtgaaaaaaggtcaggggccg
catcaaccccaacaaatctaatcctgttcgaccaacctcatacaacatat
gtctggcctactactctttttcgcacgggtagatatcctaacacatcaca
aacct

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