GrainGenes Sequence Report: BQ166381
Sequence
BQ166381
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ166381
NCBI UniGene
Ta.34779
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001051405.1 Os03g0770900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL 4DS
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0821-0824_N03_N03ZY Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_N03_N03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0821-0824_N03_N03
Probe
WHE0821-0824_N03_N03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttcatggacaaaacagggaaactgacataacctgctggtgtgagagttc
ctggccaaccccctccttagtgtaaattctagatgacaaaactcacaaaa
ccagttcgacgccatagatacacattcagtcttttggatggaacagacaa
tataatcgttgtcttctttcatattttttgccacatgttctagtcaagct
gctgctgcttggagcctggatgttttcatcgaggccttcctcttttctcc
agtttgagagggtgctgtgaaaccactgaacacagggatatgaaaggata
tcatcatgtgctaaaattcagaaatcaatgtgtaaattcagaaattcaga
aaggggaaggaactgatttggttttgcaaaccttgttattttggctgttc
ttgttgatgacgctgccctctgtgatttctctacggttttgcctttagtc
agcgcaggcctcacattgttgggccggatgctactcaaaacagttttaga
ttca
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ166381
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||