GrainGenes Sequence Report: BQ166400
Sequence
BQ166400
Contig
Ta.9609.1.S1_at NSFT03P2_Contig11501
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC367461
wEST map position
BQ166400
NCBI UniGene
Ta.9609
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001056219.1 Os05g0546600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL 6BL 6DL
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0842_B09_C18ZY Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0842_B09_C18, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0842_B09_C18
Probe
WHE0842_B09_C18
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cggcaagcaaacaaaaaaatccagatatcacaaaatctatgcatctacaa
gctaaagaaagtccactcaatgaccacaagaatccatctaggaaggaacc
atcatcgacagaacaaaagtgttatctcaacggtccatcaactatccctg
gcagctcaaaagcaaagctccgacgacgacgcgctccacatatgcctccc
tcctccggtcagtccgtcgagttctcatggagcaacacgtactcccggct
actgttgccaaacttaatgttgtccctttcaaagagttcatagtagcggt
gggactcaatgcgatcctcattgatgaaagtcccattggtactaccaaga
tccatcagataaggcctcacttgctttgacatcatgccatctggttgctc
cttctctataagtctatattgaagaaccgcgtgttgcttgctgcaggagg
gatggtcggtggggatgtctgcaatcctcctttcccttccaaaaaggtag
tggctcatgcgatgaacatacaacggttcattcagtgcttcaccaccctt
gaatacatagagtctccatctaatatctgatttgcgagcctctggaggtt
ctgaatacacaaagttatacctccaactttgttagtctcctcagcaagct
ttccagacaactcaaatgat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ166400
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||