GrainGenes Sequence Report: NSFT03P2_Contig6242
Sequence
BQ166406
Contig
NSFT03P2_Contig6242
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC283083
wEST map position
BQ166406
NCBI UniGene
Ta.38934
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1BL 2BS 3DL 4AL 6AS
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0844_H02_P04ZY Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0844_H02_P04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0844_H02_P04
Probe
WHE0844_H02_P04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
taggtcttaaggtcatacttacggtgttacatacttttagtcacatccca
tgcacataaatcgctggttaaaaccagttttctgcgcccaggggattttt
caagcgatgcaaactgaaacctcagtccttcatttgcgcaactactaata
gcacgatgacagcctaaagatttggggagccagcagtcgggcttttcagg
gatcctactttcatgctcatgttcaggtcatcaatctatgatcacgtcac
acgcgaactctggaacgtctcccttgttccccttcatggtcacaatttca
taggcgagctttgcgcgcagaccatcaatctggtcctggcttagtgttga
gcggaggtacagcccggtgaagtcagtgtagtagtgtgtgatgtacactc
cgctgtcttccctgctgcacggttcatgctgcgccacctatgggtatagg
aatttccacccatctgtgtcaaccaacccgctgccgaaaagttgttgaag
agagagcagaacttcgctgcaacttctttgcatggttctcgtgttttatt
ttcatctcatccatgacatcggtctcg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig6242
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||