GrainGenes Sequence Report: BQ166557
Sequence
BQ166557
Contig
Ta.6247.2.S1_at NSFT03P2_Contig15228
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC288049
wEST map position
BQ166557
NCBI UniGene
Ta.55075
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001062685.1 Os09g0252100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0929_H03_O05ZT Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0929_H03_O05, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0929_H03_O05
Probe
WHE0929_H03_O05
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttgaactaacattgttttattcggaaattattataa
gcttataacatccaagatagctacaaagaaaaatggagtaaactatagta
caaaggtcgcccctggaaactggcccaaaattgcaaacacaaattactgg
gtacccaaattacttgtcagacgcctatcggaacaattttacatgttgac
acagtatcacctgaaatagggccactgccaacctttatcttgtatatgct
caatgaacctaaccacgcactatcttatcttttggttgttacaggatttg
gttgcacttttacg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ166557
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||