GrainGenes Sequence Report: NSFT03P2_Contig6442
Sequence
BQ166559
Contig
Ta.10785.1.S1_at NSFT03P2_Contig6442
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC350538
wEST map position
BQ166559
NCBI UniGene
Ta.48513
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001056857.1 Os06g0156900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AS 7DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0932_D09_H18ZT Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0932_D09_H18, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0932_D09_H18
Probe
WHE0932_D09_H18
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttgaaaaaagaatgagctgctttctagtct
agcaccgtcttggcggaaagcaaatgcatagtgtgctacccaattcaatt
tgtacctagcgcacccttctatacaccaaaacctcctcgcgcgcggtaaa
tgatacagttgtggttaaaaggcaggtaagaatgtgcttgcagctgcgtg
gtgtggcgcctgaaccgcacgcagcggtagggttcctcgtcatcgtgctt
ggtggtatgcttgcaaaacgggaatgattgtgctacctgatgttcggatc
gaatcagatgtgcaggcactccaagaatctattggggt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig6442
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||