GrainGenes Sequence Report: Ta.26292.1.A1_at
Sequence
BQ166727
Contig
Ta.26292.1.A1_at NSFT03P2_Contig9692
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC339429
wEST map position
BQ166727
NCBI UniGene
Ta.26292
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001132510.1 hypothetical protein LOC100193970 [Zea mays]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3BL 5BL 5DL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0924_D07_H14ZT Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0924_D07_H14, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0924_D07_H14
Probe
WHE0924_D07_H14
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttgcacccagcaaaaccacttgcat
aggataggtctcaaatagagtttaaggtaacaaagacagagtcatagata
tcctggctactacgaactgaacaagataactacgagcaacaggctccacg
gcaaaacagagtcatcgccatctcaggctctctcacacataactcaggct
agggtaggtcgcacaaggaacagctccctccacaggagctcagcagtaga
acatgacatccttgacgttgtccttgatctttgggagctggcgcacctcc
acggggccctcgcgcgcaatcgccgcctgcccgctgctcccaggggttga
cccgccgttcgacctgtccccttcc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.26292.1.A1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||