GrainGenes Sequence Report: Ta.13208.1.S1_a_at
Sequence
BQ166913
Contig
Ta.13208.1.S1_a_at Ta.13208.2.S1_x_at NSFT03P2_Contig14985
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC294371
wEST map position
BQ166913
NCBI UniGene
Ta.13208
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001059140.1 Os07g0203700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL 2DL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0941_H07_O13ZT Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0941_H07_O13, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0941_H07_O13
Probe
WHE0941_H07_O13
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttcaggatgtctcaacgttcgtcaattctgagg
atgttctgttgtcaaattttatgtacaaagacttcggtactttacaacgt
ctatgcagtattataaatagggagacgcaggtcacggcagggtattgact
gttacacgctaacatcacctcagcaccaaatgtgtactaccttggtccgg
cggtattttctgcaggtcacggcagggtattatctgcagtaccaacgcac
ttgtcgatttgtcagcagaatggaaaacggtatgtccagtggtatgagtg
aaccaagttggttgcagaaaacctcagttcagcgcatagtggacccgctc
caacattcgcatgacctggaaaacggggatagacctgtgtggtgccttta
ctgaagacccatcatcaagaacagtttgctgttgctcactggtgagctct
aagaattgcaccagcatgtcaccatccagaattgaaggcact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.13208.1.S1_a_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||