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GrainGenes Sequence Report: Ta.4549.2.S1_a_at

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Sequence	BQ167230
Contig	Ta.4549.1.S1_at Ta.4549.2.S1_a_at Ta.4549.3.S1_a_at NSFT03P2_Contig14055
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC284492
wEST map position	BQ167230
NCBI UniGene	Ta.4549
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_973963.1 SPla/RYanodine receptor (SPRY) domain-containing protein [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Cheyenne
Chromosome	6AL 6BL 6DS
Clone Library	Cheyenne wheat endosperm cDNA library Wheat endosperm cDNA library
Tissue	Cheyenne endosperm Endosperm
Developmental Stage	5 -30 days post anthesis seed 5 to 30 days post anthesis seed
Data Source	genbank Release 136, Jun 15 2003 genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0057_H11_P21ZK Cheyenne wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0057_H11_P21, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0057_H11_P21
Probe	WHE0057_H11_P21
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Stratagene's KS primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA	ttcggacgagctgaaatgatggtcttagccacagaaaaatattgcgtcag ctcatcatgaatggggatatcgattctgcattcaaaaggcttgaagagtg gtatccacaggtcttaaaggatgacgtatcagttatttgcttcttgcttc attctcaaagattcattgaatacatcagggctgagcaactggagggtgct gtaaaatatgcccgcgctaacctggccaactttttggcacacaaagcttt tgaggggttgctgaaggagagtgtggccctgcttgcgtatgagaagcctt cagaatcttgcattggatatttgctggagtccccacagcgtgaatttgta gcggacgcggtgaatgctgccatcctgtcgacgaaccccaaaatgaagga tccagagagctgcctttactcgtgtctggagaagttactgaggcagctca cggtatgcagctctgagctacgtgcgttcaacagcgatcaaggggatgtc ttcttacttcataaggagatctacgaaagatctaggcgtccctaaggcac gtcgagcatacatacgagcagtcaactcgcaaaataagatcctcattctt cttggaa

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