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GrainGenes Sequence Report: NSFT03P2_Contig12205

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Sequence	BQ167520
Contig	NSFT03P2_Contig12205
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC325852
wEST map position	BQ167520
NCBI UniGene	Ta.63384
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title '5a2 protein'
Keyword	EST
Species	Triticum aestivum
Cultivar	Cheyenne
Chromosome	1AS 1BS 1DL 1DS
Clone Library	Cheyenne wheat endosperm cDNA library Wheat endosperm cDNA library
Tissue	Cheyenne endosperm Endosperm
Developmental Stage	5 -30 days post anthesis seed 5 to 30 days post anthesis seed
Data Source	genbank Release 136, Jun 15 2003 genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0071_E01_I01ZK Cheyenne wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0071_E01_I01, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0071_E01_I01
Probe	WHE0071_E01_I01
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Stratagene's KS primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA	catttcttcattacaaagtggcttaacattaaatttttctgagcacagcc tcacgaattacagatctgaaaggaaatcttatttgtgaggccaggcacat tggaatgtaaaaaccaacgcttctccttcccgcaacttatactagggtca ctacgcagaggggggaatgtgtaacttccgcacttgtaccatgtgggaat ggcttcttgcagaaattggcaacgtaggcaaccttctccatacaatatat cttctctttctccttggtgacaccagcgcaaaggcatgggatgtttgcct tctgccacacggcgcagcatgcgtccgacggatctagcttcgggtttgct ggccattgttgatacttagcacactccttgatcatgggctgtcggtcacc ctcacattcgtcagccgacacggctacccaataagtgaaggatgatgaaa cataagcacatcagtttcgccatggctatgtcttttgtatctgctacact agagccttctctatctctttgat

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