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GrainGenes Sequence Report: BQ167727

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Sequence	BQ167727
Contig	Ta.1305.1.S1_a_at Ta.1305.1.S1_at Ta.1305.1.S1_x_at NSFT03P2_Contig10419
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC286187
wEST map position	BQ167727
NCBI UniGene	Ta.54611
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_172848.1 EIF-5A (eukaryotic translation initiation factor 5A-1); translation initiation factor [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Cheyenne
Chromosome	5DS
Clone Library	Cheyenne wheat endosperm cDNA library Wheat endosperm cDNA library
Tissue	Cheyenne endosperm Endosperm
Developmental Stage	5 -30 days post anthesis seed 5 to 30 days post anthesis seed
Data Source	genbank Release 136, Jun 15 2003 genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0089_B12_D23ZK Cheyenne wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0089_B12_D23, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0089_B12_D23
Probe	WHE0089_B12_D23
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Stratagene's KS primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA	cgattcggacgagaacgtcgcgcccgcgaactcctcacgagagaattttg ggtcctgatccgaggagaggagatgtcggacagcgaagagcaccagttcg agtcgaaggcagacgccggcgcttccaagacctacccgcagcaggccggc accattcgcaagaacggctacatcgtcatcaagaaccgcccctgcaggtt gtggaggtttcaacatctaaaaccgggaagcatggtcatgccaagtgcca ctttgttgctattgacatcttcaccggcaagaaacttgaagatattgtgc catcttctcacaactgtgatgttcctcatgtgaatcgcacagaataccag ctcattgatatatctgagggatggatttgtgagtctgctcactgataatg gtaacaccaaagatgacctcaagctcccgactgatgaaaccctccttggc

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