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GrainGenes Sequence Report: BQ167975

Sequence	BQ167975
Contig	Ta.27384.1.S1_at NSFT03P2_Contig13887
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC308909
wEST map position	BQ167975
NCBI UniGene	Ta.27384
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wdk3c.pk006.c16:fis, full insert mRNA sequence'
Keyword	EST
Species	Triticum aestivum
Cultivar	Cheyenne
Chromosome	3AL 3BL 7AL
Clone Library	Cheyenne wheat endosperm cDNA library Wheat endosperm cDNA library
Tissue	Cheyenne endosperm Endosperm
Developmental Stage	5 -30 days post anthesis seed 5 to 30 days post anthesis seed
Data Source	genbank Release 136, Jun 15 2003 genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0023.C09F990702ZK Cheyenne wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0023.C09F990702, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0023.C09F990702
Probe	WHE0023.C09F990702
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Stratagene's KS primer. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab. Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA	gattcggcacgagcagccatgacaatcaccacctcgagcttcctcaagac ctcattccctggcgtttgcattccggcagccactcgcaccccctcctgtg ccgccacccggcgcaccggggccatctgcaactccatctcctcctgcacc cctccctatgatctcagttccttcaagttcagccccatgaaagaatctgt cgcctcccgtgagatgatccgacggtacatgaccgacatgatcgccgatg tcaacaccgatgtcatcatcatcggaactggcagcgcgggactgtcctgc gcctacgagctctccaaggacccgtctgttaacatcgccatcatccaacg gtccgtttcccctggcggcagcggctggctcggcagccagctcttctccg ccatggtcgtgcgcaagccggcgcacctcttcctcgatgaactcaacatc gagtacgacgagcaggaggactacgtggtcatcaagcatgctgcgctctt cacctccaccgtgctgagccgcctcctcgcgcggcccaacgtgaagctct ttaacggcgtcgtcgtggaagacttggttgtcaaagagcatcgcgtc

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