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GrainGenes Sequence Report: BQ168177

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Sequence
BQ168177
Contig
Ta.28205.1.A1_s_at
Ta.829.1.S1_at
NSFT03P2_Contig17980
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC280758
wEST map position
BQ168177
NCBI UniGene
Ta.54449
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_565289.1 ATUBC2 (UBIQUITING-CONJUGATING ENZYME 2); ubiquitin-protein ligase [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Cheyenne
Chromosome
2AL
2BL
Clone Library
Cheyenne wheat endosperm cDNA library
Wheat endosperm cDNA library
Tissue
Cheyenne endosperm
Endosperm
Developmental Stage
5 -30 days post anthesis seed
5 to 30 days post anthesis seed
Data Source
genbankRelease 136, Jun 15 2003
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0040.B11F990526ZK Cheyenne wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0040.B11F990526, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0040.B11F990526
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Stratagene's KS primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, Cheyenne endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
cgattcggcacgagtaacctgcccgaagcgactggcgggtagagtgcgtg
cgtacgtgagccctagcgggttcgaggaggcagcgccggcggcggagatg
tcgacgccgtcgagaaagcgcctgatgcgggacttcaagcggctgcagca
tgacccaccgggcgggatcagcggcgggccgcacgacaacaacatcatgc
tctggaacgccggcatcttcgggccggatgatacgccatgggacggaggc
acagtcaagcttaccttgcagtttacataagattaccccaacaagccgcc
aactgagcgtgttgtctctaggatggtt

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