GrainGenes Sequence Report: BQ168352

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Sequence

BQ168352

Contig

TaAffx.91297.1.A1_at

TaAffx.91297.1.A1_x_at

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ168352

NCBI UniGene

Ta.50998

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

7AS

7BS

Clone Library

Wheat salt-stressed crown cDNA library

Tissue

Crown

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2102_D03_G06ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2102_D03_G06, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2102_D03_G06

Probe

WHE2102_D03_G06

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttttttttttttttttttttttttttttttttt
ttttttgggacagacaacaaaacattgcattaaactctgataaaaaataa
aaaatagggggaggggtttattccacccaaaaaatgggggtggaatgaac
caacgaacactgcccaaaacctcaaaattgtgccgagacaacaatttaac
acaccccacaaccaaaccaaatggaaactacggtagacagaaaaatcaaa
acccagccgaaacttcacccccccgctaaatgcccctcttcatgccccat
tgcacccccctatttgtcggggctctccccatcaactgccaatggaactg
gcaaa


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