GrainGenes Sequence Report: BQ168367
Sequence
BQ168367
Contig
Ta.6868.1.A1_a_at Ta.6868.2.A1_x_at NSFT03P2_Contig8794
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ168367
NCBI UniGene
Ta.6868
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049885.1 Os03g0305700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS 4BL 4DL
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2120_B05_D10ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2120_B05_D10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2120_B05_D10
Probe
WHE2120_B05_D10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttaagacaatgtaagatgtagctgtagcca
ccattgtgaaatgtaagtcatgtcagtagcattcaaatggatgctgctgc
gttcactcgtacgcatgcttcattcttcatctccagcaagagcaacagaa
agcttacagcttatttcagacggcaatgcaaaaaagaaaaaaagaaaaaa
ctactactttggtaagcagagtggcactatgaatactgtaagtactgata
ctatgaagtacagtacaagaaaatactgtatatggtcgcctccatgcatc
tgaacatgagcaaaaaaatggcaccttatcccagacaccgaacctttcgc
cactcaagatgtccattgtatttgtcagtgtagtaaagaaaataatagcc
cctcttactgtgagttgggcactgtcacggtctgcttgtgctaacaatac
atgatagacatggtttgtgttcagacactttcatcaactttatccaatga
gaatgacaaaaagctcagtatgaaatcatccaggtcgccttccaggactg
aatcgggatcagacacttcatagtttgtccg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ168367
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
| |||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||