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GrainGenes Sequence Report: BQ168436

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Sequence
BQ168436
Contig
Ta.11045.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC354935
wEST map position
BQ168436
NCBI UniGene
Ta.11045
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2106_F05_K10ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2106_F05_K10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2106_F05_K10
Probe
WHE2106_F05_K10
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttagcaccagtttgatgtatt
cttcaaattcatgcatcaaagtgaacattgatggcacgttcagacactac
tggtataattacctcattacagaacggatgcaggatgagcttgactacta
aatggagagatcacaagtgacacgcctaagatcagtgccaatcagaacca
cggttatggttccataattacaagataaacggcttcctgtaaatgtcgaa
tgcttcggacgcgcacagctgaggctcctgtgcgctgctga

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