GrainGenes Sequence Report: BQ168445

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Sequence

BQ168445

Contig

Ta.11050.1.S1_at

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ168445

NCBI UniGene

Ta.40515

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, weakly similar to NP_001067535.1 Os11g0225000 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4AL

4DS

Clone Library

Wheat salt-stressed crown cDNA library

Tissue

Crown

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2108_B03_D06ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2108_B03_D06, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2108_B03_D06

Probe

WHE2108_B03_D06

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttttttttcaagaaatttaaaattcttatttca
aaccatgcccaacagaaacaacaacattgcatttnggagtataaaaccat
ggactacaacagataatggagtaacagttaccataataaatttaacaagt
tccacaatgacgtaacaacacaacttattatttcagaaatcaattcatta
tagtaataacagaggaagttcttttctttccatggtgccgttgtttcatc
aagagaagatcctagtttcatgcttgcatgggagcgttagaggcctggtt
tgccattcagtttgcacagcgtccttcgagcctcttaacaatattttcaa
tggaaggtctcttcttccgatcaatctgcacacactctagaccaatttta
atgcatacttttatttc


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