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GrainGenes Sequence Report: BQ169412

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Sequence
BQ169412
Contig
Ta.11116.1.A1_at
NSFT03P2_Contig11968
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC307928
wEST map position
BQ169412
NCBI UniGene
Ta.11116
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054941.1 Os05g0218400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS
3BS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1755-1758_K23_K23ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1755-1758_K23_K23, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1755-1758_K23_K23
Probe
WHE1755-1758_K23_K23
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttttttttttttttttgcgtc
caacaaaagaactatatatatatatatctcaagaacaaaatatcattttt
gttcgttgcaaccaagtcagctggcagcttatgtgaattcagctatgctg
tggatttacaaatgatgtcctgaggttaactctctccaaggaaagggaaa
gtggtcatctcatggaagcctggctctcgacgtcgccgtcgtctcgcttc
gtactagtccccaactccacctcctgcgtctgctgatggccgtcgctgct
cgggacggacgggttctggtgctcctgctcgtgctcgccgctggacggtg
ttagctggctgctctggaagctgctggtgccgaacgccatcttcctgaac
ttcttcaggtcctcgttgtactggctggtgtcatactcggagctggcgtg
cgatcccatgaaccggctgtggccaggccgga

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