GrainGenes Sequence Report: BQ169498

Sequence

BQ169498

Contig

Ta.11151.1.S1_at

NSFT03P2_Contig8935

External Databases

TIGR Gene Index

wEST map position

BQ169498

NCBI UniGene

Ta.11151

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001051707.1 Os03g0818300 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

5AL

5BL

5DL

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2339_D02_H03ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2339_D02_H03, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE2339_D02_H03

Probe

WHE2339_D02_H03

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttggaaattgcctaccaaggcacaattcaatca
tcatcagcaagcgttgcaacgacattgtccacacctggggaatgaacaca
tcaaatgacaaccaaggaaacatctataatgctacgagagtatgagctat
gtatcagtctttcacagcacctacggctaggagactaccacatgggaggg
agcacagtttctagaggaatccacatccgctagccgcgaccttgggcaga
aaagaaacatggtgtagtttgatcagatgggtcagagacggctgaagcag
acttgcaggcgacggcctactgcctcattttcttggagctgctgcattca
gggcacttgtagtgctttatgtgatccgctttggccggggttatccgcac
gcatttgccgtggaaccaccgctcgcacatgtcgcagccgatccagaact
cgcttgcattgtatatgccggtgcagcttccgcaaaaggtttcgctgtgt
tcttcttcgtcctcgtcctcgtcttcttctcgagcaactgccctggagtt
ctttgggcggacatcatcagttcgctttggctttgatgagtgcctggatt
tgccactgctatca


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.