Query (optional)   in Class  

GrainGenes Sequence Report: BQ169852

[Submit comment/correction]

Sequence
BQ169852
Contig
Ta.2959.1.A1_at
Ta.2959.1.A1_s_at
Ta.2959.3.A1_a_at
TaAffx.51539.1.S1_s_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC354371
wEST map position
BQ169852
NCBI UniGene
Ta.65941
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054160.1 Os04g0662900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS
3BS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0973_H07_P13ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0973_H07_P13, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0973_H07_P13
Probe
WHE0973_H07_P13
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttagaagagtcagttaatggctcaaattcaa
gaatccaatttacatgcataagactgcaaactgaagccatattcctggta
aacatgtctccagagagcaacccaaagggacaaactatgccagttcaagt
ttggcagactgaagctaaatcttgtatctacaaacatggacgataaacaa
tagaggccaaaagtgtatgcgctccaaacagacaaacaaaatcacactaa
aagttcctagagggaactgccgccatacgtagtttcctaccgggaactgt
cgtcatacgagctgcatattacacaagcacgtcacactggttggttggtc
atgttcctagccgaacagagtcagtcagatgtccatgtcgatcaacgcat
cttcctcctcgccatccgagagtgca

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.