GrainGenes Sequence Report: Ta.11398.1.A1

GrainGenes Sequence Report: Ta.11398.1.A1_at

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Sequence

BQ170012

Contig

Ta.11398.1.A1_at

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BQ170012

NCBI UniGene

Ta.29542

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4BL

4DL

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0986_H12_P24ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0986_H12_P24, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0986_H12_P24

Probe

WHE0986_H12_P24

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttttttttttttttttttttgttcataaaaagacaatgcatttaaaaaaa
accaaatttgcatccgcagtttaaaaaaaaatgcacaacatgtacatcta
gtccctaggaaaaacataggtttgcacattcttctctatacatggcacat
atcttgtttctctgttcgactgtacttagtctgcatcttggcatcctaaa
atctgcagtcttaggggctgtaaaaggcagaagcaccattccaaggtgtg
cgataggggggggtgtatgaagtgctcgaagagacaagtgcaaaggatgg
agatgctcagggagggaggctgtgatggggaagctaagcgccgcacagca
tcatgccgagctgctgtagcctgtggaacgtgcagctatcggagctggtg
gtcaatataccaaagacgagaacccacggtgcggcccctagcgatagcaa
catccccatccatgactagtgcccagctcaccacgcacaccttagtgggg
aagatggatgcagagcaccaggggaggtggcgtcctcagaactgcgaccg
ttgatgcctgatccacggagggcgcgcgagctgga


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