GrainGenes Sequence Report: BQ170142

Sequence

BQ170142

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

TIGR Gene Index

TC282855

wEST map position

BQ170142

NCBI UniGene

Ta.37734

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001048765.1 Os03g0117100 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4BL

4DL

5AL

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0995-0998_H05_H05ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0995-0998_H05_H05, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE0995-0998_H05_H05

Probe

WHE0995-0998_H05_H05

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttttttttttttttttttttaccaaaagcacca
tgcactaacacatgatcgaccatacataccatgtccattagtactgttca
tagcataccgtgtacatgtatatgtataaaaaccgtacgtacagacgatc
gacatcatcattctatatatagtgctacgtacacaggaataaaacatctc
agattccatgatcgagcacagcatcggcttgagttaagcggaggcagcgt
ctggactagcatgcatggtgtagcgtccgttactcacgccttgagtttgg
ccaggggagcgcgggacgggagttgctggt


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.