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GrainGenes Sequence Report: Ta.10532.1.A1_s_at

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Sequence
BQ170255
Contig
Ta.10532.1.A1_s_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC292580
wEST map position
BQ170255
NCBI UniGene
Ta.41930
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Pore-forming toxin-like protein Hfr-2'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4BL
4DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1759-1762_L06_L06ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1759-1762_L06_L06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1759-1762_L06_L06
Probe
WHE1759-1762_L06_L06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttttttcaccaaataaaaatgta
tatagtgggtaaagggaaacatataagagcacacaacccacaaactaaaa
aataaaatgaaagtcacaacacaccatcaaatcaccacacagaaaagccg
ggccactccaaggcagggatggctttatttaatacggctaactaatgcat
gcatcgtcacttcaaatgggctcttgtgtggcttcaaattggaaattata
gttgttcataccagtgaacagaccatcatccatcttgtaggtaacaacct
ctcctgtggtcagaatgtccctctgagtgtaggagaacgggacgtcaaca
gaaccctgtgtcacagccgctctgactgtcaccttggtcatcggaggcac
cacagcatggtagggaggcctcttgtgaggccgttgtatgaatggatgac
ccccactcgtattctgaattgaattcagtggatacctcaaccttcccttc
tgctatgactgg

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