GrainGenes Sequence Report: Ta.11667.1.A1

GrainGenes Sequence Report: Ta.11667.1.A1_at

Sequence

BQ170568

Contig

Ta.11667.1.A1_at

TaAffx.84119.1.S1_s_at

External Databases

TIGR Gene Index

wEST map position

BQ170568

NCBI UniGene

Ta.40233

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001068027.1 Os11g0536800 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

1BS

3BL

3DL

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1788_F06_K12ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1788_F06_K12, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1788_F06_K12

Probe

WHE1788_F06_K12

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttgtggctaaaagaatcctgccaatattttggcca
tgactaaaaacttggtctttgcagagacggatctgcatagagctcaacaa
gggccagggccccccactaaagtctacaaatctcacgagagtttgcttgc
acttttcaattaaaaatgcttaaagttttcagatctattacacaagaagt
accatcactccactgatttgcccccactccagtttgcacttctctccaat
cagtggcagaatttgtggcgtgtcactccagcgagaatccgtgcatcccc
caatgtaactcacaatctatggccctgccgccgtagaaatatttcatttg
tctcaagggaaacagcacacggagttttcttctcaataccatttcatcag
ccggctaatcacttgtgtattttctagtttgcgtca


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.