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GrainGenes Sequence Report: Ta.5965.1.S1_at

Sequence	BQ170678
Contig	Ta.5965.1.S1_at NSFT03P2_Contig12131
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC284146
wEST map position	BQ170678
NCBI UniGene	Ta.48469
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5BL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1795_D05_G09ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1795_D05_G09, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE1795_D05_G09
Probe	WHE1795_D05_G09
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttttttttacgggtctaaaagacgacggcaaac ataattaacacccagcaatgttcaatcccaaacatataacaggctggctt tcaaacatacgattacaggatccataatatgtatcgtttactctaatgga tattcattagtacatcatcttcatcacataaatatgatattttcccacat ttctgtctgcaccgcaccaaacatcatcttcggtttgagaatcccaaaag ttgcacctttgacaaacatcatcttcgatttgagcgaaccatactcccaa gtctttaccttgcaaacttactcttctttgaaggatgaggtgcaagcttg tcgttgttccatagcatccctgttggcctttcaaggtgtctggagaaaac agctggaagtaggggcacattancaaaacggcttttgcgagcttgcacat ccacacccacagacttagcatagcaggactgttgattgatatatggagta catgactgaacatgtgcctgtttcatgtcaaccatgatcatc

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