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GrainGenes Sequence Report: BQ170749

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Sequence
BQ170749
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC359662
wEST map position
BQ170749
NCBI UniGene
Ta.54772
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001049014.1 Os03g0157400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL
3BS
4DL
6BL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2301-2304_G05_G05ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2301-2304_G05_G05, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2301-2304_G05_G05
Probe
WHE2301-2304_G05_G05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttgggaaaaaaagtccatcttctcgtg
gcgcaaatataaatacagacttccctacatgcaatcaaaaacagccggta
agaactaggagcgccctccagttcacttgacctgctagccgttcgaagcc
ttcactcgcctgagggacaggtaggccagcacccggtacccgatgatcat
cgcgaccagggccccgacttcggtcacgccgttatccagaggtacggagg
tcatcaggatgtccggcacggggtcgtactgcaccttcagcagcagcctg
tacgtgtggtagttgaaggacaggtaccggagccaggatatgaatggcgg
aaccctctttacaaagaaccctcctgctagcatgaatgtcatgactgtga
ctgaagctagagtagtcgccttcttgatatccaagagggtagctccaatt
gccagtcctagtccctgcatacaacccccttcttgagtcatagatttcaa
atcagtgttaatgttcttttgaggcatcatgtaatattatcatgtagatt
aggaaaccttttccc

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