Query (optional)   in Class  

GrainGenes Sequence Report: TaAffx.128854.1.S1_s_at

[Submit comment/correction]

Sequence
BQ170763
Contig
Ta.12621.1.S1_at
TaAffx.128854.1.S1_s_at
NSFT03P2_Contig11821
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC351421
wEST map position
BQ170763
NCBI UniGene
Ta.41042
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001060760.1 Os08g0101400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AL
7BL
7DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2301-2304_L01_L01ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2301-2304_L01_L01, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2301-2304_L01_L01
Probe
WHE2301-2304_L01_L01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttacatcactcaaaaatctccatccaattgtcc
ctctcgtcgaaaaggatggaacatgttcatgtatgtgcaccacccacaaa
ccgttcacgacggaagaaactctacacttttcacctcgagagtcgaggtt
tggacaaaatgtaagatacacgaaacatgtcaacttggttcggttcatgc
agcatgagttactctcaggttttcgacttggcagggaacgcaaacataaa
tccaactagaagagcgaccaggagcacaaacaaggcagctaggatgctga
aactgttgccctgcgttctccctgaacccgtcgcctgttcagggcgcaca
tctttcagactgggccccgcccgcgcacgtgacgaagctccaggcaccac
atcccaaaacgattctagtgtgacggtagatgatgcggtttctggttcta
ggattgagttctctacttctgtttccagtgatatcacttccttgcagcgc
tccattgcatcctttgtagtttctgattgttgcaagtagtcacaaata

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.