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GrainGenes Sequence Report: Ta.28203.2.S1_s_at

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Sequence
BQ170889
Contig
Ta.28203.2.S1_s_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC328873
wEST map position
BQ170889
NCBI UniGene
Ta.29249
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2DL
3AL
3DL
5DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2311_D07_H13ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2311_D07_H13, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2311_D07_H13
Probe
WHE2311_D07_H13
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttggaacaaccgaaacacattcagtatagcaacg
acaaatcacatcccagcaataatgaaatatatgaggcacgcatcactgat
tgttaaaaacccacaaagttgctggggcagtggcagtggtaaccacaacc
aacagagtttatgcgaacactgtcattgaattctcacactatagaaagac
gaaatactaacaaggaaaactaatttcatgtttgacaaggtacttcaaag
tctgctcctgagagtaacttcaccatcccagtcgcacctagagctcgagc
ttgagctccgtcatgccgggctcgcgggtgaggt

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