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GrainGenes Sequence Report: NSFT03P2_Contig17910

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Sequence
BQ171138
Contig
Ta.11952.1.S1_at
Ta.11952.2.S1_s_at
NSFT03P2_Contig17910
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC292261
wEST map position
BQ171138
NCBI UniGene
Ta.11952
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001052131.1 Os04g0165300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AL
2DL
6AS
6BS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2335_E02_J03ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2335_E02_J03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2335_E02_J03
Probe
WHE2335_E02_J03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttacaaaaaggaatcaagtctttcaatggta
tatacatacagaactactcatggcaaccaacagacaagcaactcgcctta
ttaggatcacaagactatgaacacggcatggcaagataaaatagatatgc
agagttactaaaagttctcaggatcacttagcctgtgttgcataagtaga
tggttggctcgtgggaatttccctattttcattcttggggtgcaagggta
caaaattacaggtatgatcaaccttgtattgaactgaagatccaccatgt
acagttaactcatttagttgagatgaagtgcacaagaattttttgatcga
caactttgaggtcagttcagattgacccttcagaaaagcactaacgtgtc
tgggtcacttaaaatttaaattgatataacgctg

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