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GrainGenes Sequence Report: BQ171203

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Sequence	BQ171203
Contig	Ta.6080.1.S1_at Ta.6080.2.S1_a_at NSFT03P2_Contig15695
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC298839
wEST map position	BQ171203
NCBI UniGene	Ta.54580
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001130889.1 hypothetical protein LOC100191993 [Zea mays]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6DS 7DL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2339_H03_P05ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2339_H03_P05, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2339_H03_P05
Probe	WHE2339_H03_P05
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttagacaggcaatacactaaacctataata aacctcaaaagcaaatccattctcnggatatctatcagttacagcgagct gatcacaccgaagccatggcagtatctcccaatcaatcaacaacaggctt atgcaactcaacacgaccatgggtgaatggcctcggatttggagctcagg ctttactaatcactattaatcatgaaaagcgatggaaaaaaaaagcatct ctcaagccgcagccgacttgcgcagttcactctcaccatgggcgaagtgg catctgtctccaaatgtgcaagagcctttggtgaagttgtcacacagctt ggtcttgaagttgcttcccccaccaccacccgcaggccccctccctgggt tctttgcttgaggaggggcgttaccgccaatgcggagaatcagctctttc accattgcacttgcatggttgacctgatcaaacgacccctcaagctcaat gtttttatggttggggtctgcctcgttatccggatggccagcttggcccc agtca

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