GrainGenes Sequence Report: BQ171345
Sequence
BQ171345
Contig
Ta.4230.1.S1_at NSFT03P2_Contig10228
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC300400
wEST map position
BQ171345
NCBI UniGene
Ta.44161
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001055289.1 Os05g0355300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1BL 1DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0974_F05_L10ZY Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0974_F05_L10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0974_F05_L10
Probe
WHE0974_F05_L10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gcctccatgccatagtgaactgtgctagactgcagcaactgtaattttca
actttactatataaatatttatttatatgaatggtttacaactgatggaa
ttgattgaacattgccccctgcaatacagtatgtgcagtattcattgaat
gctaattacaagctgctgctgctcctggctacgcctggatgcattccttc
ttctttgctcctcaagcagctgatgcaaccggtactggcgccgcccgtgc
tgaatcatgcggatcaccctcatgacggcgtagagcggcagcaagatccc
cgcggcccgaagaatgtacatcgtgaggaggctgaaggcacagtgctccg
cggccttgatggtcaccacggcgatgagatgccacaccagcagtatgatg
gtgaactgcgtgcacagaagtttc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ171345
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||