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GrainGenes Sequence Report: BQ171559

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Sequence
BQ171559
Contig
Ta.12091.1.S1_at
Ta.12091.2.S1_a_at
NSFT03P2_Contig7408
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC288131
wEST map position
BQ171559
NCBI UniGene
Ta.12091
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
TAM W101
TAM W-101
Chromosome
7AL
7BL
7DL
Clone Library
Wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1412_C03_F06ZT Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1412_C03_F06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1412_C03_F06
Probe
WHE1412_C03_F06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttcgaaaaacagacctcgtgccacttttttattac
agtacatgcatgatacatagagtatcagaatctccgatctttgtcctcct
taacgtaaatagcagtactacgttttacatccacgtataaacgcgggaaa
catgcatatagaacacttaattcgtcaagtatacatgatacataaagcat
cacgatacgtgtagctagtctcggttgattattattattatggtcctcgc
aatttataaacgaggttaatttcgcaaaaatataccgtccacgctcgcct
taaaaaccgacgacgtcgatcgattaacaacgcgtctgctgcgcgccggt
ggcggtancggcgaaacggtaggccctggggaccccggccgcggccatta
acgatggcgccgacgttgaggccgacgttgacgacgac

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