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GrainGenes Sequence Report: BQ171602

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Sequence
BQ171602
Contig
Ta.8567.1.S1_at
NSFT03P2_Contig11621
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC284748
wEST map position
BQ171602
NCBI UniGene
Ta.54077
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001047584.1 Os02g0649700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL
6BL
6DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1659-1662_J16_J16ZT Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_J16_J16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1659-1662_J16_J16
Probe
WHE1659-1662_J16_J16
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttattttttttttttttcttttttttaaca
agaatctttcactggggagggctgaaaaaaaagatgtatgaagaaaagag
aatgttcttttacatgtgcaccacctgcctaatccctataacaacattgt
agaggagcaaacagatggacttacaatattgtggaggttagaactcgata
atcacttctggaattcctcgagtcaagggcaaccgcgaccgtatatatat
acagcatcttgggcatgaggactttttttttcactgaatccctctggatt
tacagagttttagcggttggcgctaggttaaattaatcaagtctaaaact
ccaatttatactcctgatgc

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