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GrainGenes Sequence Report: NSFT03P2_Contig8345

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Sequence
BQ171728
Contig
Ta.12169.1.S1_at
NSFT03P2_Contig8345
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC328091
wEST map position
BQ171728
NCBI UniGene
Ta.58207
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5BS
5DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1659-1662_D06_D06ZT Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_D06_D06, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1659-1662_D06_D06
Probe
WHE1659-1662_D06_D06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttggataaaccgtttggtccagtattat
tatacactcactagtttctgcatgtgtgcatgtacatgaattttttacag
gaattgtttggcctacggtcctagtagtatacaacagacacaaatcagag
aaatgtgatacagtaaataagtatctagccatacaggtgacttctacttg
cacaaatctcgctgccaataatgcttttcagtgattctgaaatgttggta
cttcaatttgtaaatgcccaccgcgaattgcaatcacggccgtaaatccc
aatccacccaaattggcctgcccggtttcaatttgcgagctgcaaatctc
agctcgcacgacgcggtgtaatcgtcgtatccatggattgtgtgcctcca
ggtttcagtagttcagtctccagatgtgctgtacaaagagagttattacc
tatgagaaaacacaagttcagtgtcttcgcttcatcaccctt

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