GrainGenes Sequence Report: BQ171864
Sequence
BQ171864
Contig
TaAffx.8261.1.S1_at NSFT03P2_Contig7581
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC296760
wEST map position
BQ171864
NCBI UniGene
Ta.40589
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001043401.1 Os01g0579800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL 3BL 3DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1659-1662_N03_N03ZY Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_N03_N03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1659-1662_N03_N03
Probe
WHE1659-1662_N03_N03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gccccttggttttggagcatcatttagtgtgcgcgagaatactccagaat
agactccaccatcataattttgcatctaaaaggcaaccgtttcctagaga
ctggagttactcaaaaaatttggctacgaaatgtcgacgtatcatcacgc
caagccatactatttgtgccagctaacagcgctagattgacaataaaata
tacccttgctggaattgcagggggagaaaagacatccaaggccagcagga
gaactgatgaaatcaccacaaacgagggagagactctgccctgatgatct
ccttgagaagagccttctcctcattgattttgctcttctcagcagcgtca
cgtggtttgctcttccgcttctcttccagcatccacttgtagaccttgcg
ctgctcatcttcggataaaggtgggagtactcttttcggcggaggagcag
gctctgcatgcttagcaactgcagcaggtggggctgacaatgctgctgcc
tcgtgccgaa
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ171864
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||