GrainGenes Sequence Report: BQ171868
Sequence
BQ171868
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC367191
wEST map position
BQ171868
NCBI UniGene
Ta.50556
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1DS 5DL 6BS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1663-1666_G20_G20ZY Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1663-1666_G20_G20, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1663-1666_G20_G20
Probe
WHE1663-1666_G20_G20
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ctttgttatattttgtcatgggccatctgctttctctgttatgtaccaat
tgttgtcgaacacacgcagcagccttatcaatgcagcacacttacatcgg
catgacctactattctccgcctctggtttcccctgtataattcatttaaa
cacacttgaagcaccgacgcccatggctgctgcttaccttccaaaacaat
aattacatgaatcagtgtttaccgagcagccgcagacgatttcttgcatt
cactttgacaccttgtcctctcgacgtttagccagctcttaccgtacctt
attctgaatctaatctcccaagaattgcagaaagtaagtcagtcatattt
taaaacccataagctcatacataatctgaactcagtactc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ171868
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||