GrainGenes Sequence Report: NSFT03P2_Contig9809
Sequence
BQ171939
Contig
Ta.2822.2.S1_at NSFT03P2_Contig9809
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC336774
wEST map position
BQ171939
NCBI UniGene
Ta.2822
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001058384.1 Os06g0682900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 7AL
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1701-1704_O14_O14ZT Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1701-1704_O14_O14, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1701-1704_O14_O14
Probe
WHE1701-1704_O14_O14
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttagttacaccgagttcaatttacgcacacaca
caagttctcgccttattgctagctgctaccaacacgctgcctcggctcca
caactcacaactccatcaacagtgcttaggttctcagtaacacttcacac
aactcacggcagtagccagtaggaactcaactccatcaacacttctcgct
tgggtctcagctgaggctcatgtcagagctcaggaaggccgtcatcacat
caggaagaacggcgaagggagcaggggccggacgctcctgcgcacgtcca
agccccggaggcgctccaggttcatcacctcggagtggtcggcgaagagg
ttcacccgcgggccgtatcgtttgacgaaccactcggaggtgttggcacc
ggacgtctcgaacatggttctctccagccc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig9809
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||