GrainGenes Sequence Report: BQ171953

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Sequence

BQ171953

Contig

Ta.13635.1.A1_at

External Databases

TIGR Gene Index

wEST map position

BQ171953

NCBI UniGene

Ta.13635

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001064574.1 Os10g0409900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

1BL

1DL

Clone Library

Wheat heat stressed spike cDNA library

Tissue

Whole spike

Developmental Stage

Spikes at 5, 10, 15 and 20 days after anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1704_E03_J06ZT Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1704_E03_J06, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1704_E03_J06

Probe

WHE1704_E03_J06

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttattcgtaattttctacacaaataagcttgttta
gtttctgatacatataaaaacccatatgaacttcaaaatgaagtaagcat
tagaagcattacttattctaatctcgaagtctggtgtggggctacaacat
agatcaacataaagcagtaaatgaatgaatacaggataaacgagctgatg
gtggatatcccaatatatatattgccagaatcttgaattgcagcttctgg
taatcaaatgtacaaagatttgttcaggatgagcatttgatcttgtaatg
gaggtgttgtttcgctgatttcatcatgtcccgattcgcaacagctgacc
tcaaatttgcagcacctgacaatggattatgtgactcttcaggttacgtc
ctgcaatggtcgaccacaggtttgccacaaatgactttgtgctgacctta


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