GrainGenes Sequence Report: BQ172086

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Sequence

BQ172086

Contig

NSFT03P2_Contig18717

External Databases

TIGR Gene Index

wEST map position

BQ172086

NCBI UniGene

Ta.28319

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Clone wkm2n.pk009.f17:fis, full insert mRNA sequence'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

6AS

Clone Library

Wheat heat stressed spike cDNA library

Tissue

Whole spike

Developmental Stage

Spikes at 5, 10, 15 and 20 days after anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1713-1716_J19_J19ZT Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1713-1716_J19_J19, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1713-1716_J19_J19

Probe

WHE1713-1716_J19_J19

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttttttttaacaaacaaagcttagtacctagat
aaaatgctaatgacacttcctctttacaatgacctgatgccaaaatcata
cattcctgggcatgcagcacggggtaattgtacaatagtcttcagtgaag
ccgatgaacttcagaccaaatatcatacatgccatgaatctaccctatct
tgatccaattagttggtgacaaaatggtacctggagtccacatagctctc
aacgtcccagaggaaggatccgaaagtgac


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