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GrainGenes Sequence Report: BQ172214

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Sequence	BQ172214
Contig	Ta.12377.1.S1_at
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC364334
wEST map position	BQ172214
NCBI UniGene	Ta.46095
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3AS 3BS 3DS
Clone Library	Chinese Spring wheat drought stressed leaf cDNA library
Tissue	Leaf
Developmental Stage	Full tillering stage
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2013_B02_C03ZT Chinese Spring wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE2013_B02_C03, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2013_B02_C03
Probe	WHE2013_B02_C03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton , Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 80%, 70% and to 60% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttttttttttggagcaataaatggaattaattgt tttaagtactcccaggaagagcaaggtatccggttaaaattaaccctaca acctggcatgacatcctcgcacgactgagaaccagaggcaaatgtagcat cagaaatgacacccgaaaactgctctttcggaacgtcggaaacactgcct acagagctgcaacaaggttgcaaagctttgggagggaggagcttcccctc accgttgctcggaacatgcagcccctcagcatggttgatgctgaggcttt tgtcttcaacatctgagcctaccttgtcttctcctgtcttgctgcagttt ttcgccaaaccaggcatgctaaaaaccactgcatgatgagactggccgtt attatctacttcatcgcgcatatcatc

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