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GrainGenes Sequence Report: BQ172348

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Sequence	BQ172348
Contig	NSFT03P2_Contig5166
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC324958
wEST map position	BQ172348
NCBI UniGene	Ta.12444
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001048181.1 Os02g0759400 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AL 6DL
Clone Library	Wheat salt-stressed sheath cDNA library
Tissue	Sheath
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2055_F10_K19ZT Wheat salt-stressed sheath cDNA library Triticum aestivum cDNA clone WHE2055_F10_K19, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2055_F10_K19
Probe	WHE2055_F10_K19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin , Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA was prepared from sheath tissue, equal quantities of RNA were pooled from the two samples, polyA was purified from the pooled RNA, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttaaaacacaagcttcagtgtcatatatcagatga ctatggaattttcaaacaaaaaagatgactagatatggaaattaaataag actgacgcagaaaaaaataattcaggtgcctccacagggctccttgagca ttctgcatgcggtgattacagtctctttcttggattaatagcacagacag attccccgcatgcgtggaagaacaccgcttctagtagattcaaaagtccc gtttcttttctacgttcgaacctgccggctagctgccttgtcgacatcgc cggccacagagccgaccttggcattagacacgccgccgcccagaggagaa ccttcgaggaaatcgacggcggccacgcgcttccctctaatcctcgtcga cgacgacgaggacgcctcccctccctcggagctttccgttctcctcggcg tcg

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