GrainGenes Sequence Report: BQ282397

[Submit comment/correction]

Sequence

BQ282397

Contig

NSFT03P2_Contig18207

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat cold-stressed seedling subtracted cDNA library

Tissue

Seedling

Developmental Stage

Five-day old seedling

Data Source

genbank

Release 135, Apr 15 2003

Title

WHE3071_E10_I19ZS Wheat cold-stressed seedling subtracted cDNA library Triticum aestivum cDNA clone WHE3071_E10_I19, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE3071_E10_I19

Remark

DB_xref: taxon:4565

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized ,germinated and grown aseptically in the dark at room temperature on filter paper with water,nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made,and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi,Close,Fenton) at the University of California,Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before subtraction was carried out. The mass excision of phagemid library and subtraction were done in HT Nguyen lab by D. Zhang at Texas Tech University. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ttcactgttgttggtgctcgcattcccaaaggtgttctgcttgtcggtcc
tcctggcactggtaaaactttgcttgccaaggcaattgcaggagaggctg
gcgtgccgtttttctcaatttcaggttctgagtttgtggagatgtttgtt
ggtgttggtgcttccagagttcgtgaccttttcaagaaggccaaggagga
atgctccttgcatagtatttgttggatgaaattgatgctgttggaaggca
aagaggtacaggtattggtggtggaaatgatgaaagggagcagactctca
atcagcttctgactgagatggatggttttgagggcaacactggaattatt
gttattgctgccactaaccgagctgacatcttggattctgctttact


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.