GrainGenes Sequence Report: Ta.20971.1.S1_at
Sequence
BQ295387
Contig
Ta.20971.1.S1_at NSFT03P2_Contig4647
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC289464
NCBI UniGene
Ta.20971
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001041856.1 Os01g0117900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Four-day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2869_D07_H13ZS Wheat unstressed root tip cDNA library Triticum aestivum cDNA clone WHE2869_D07_H13, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2869_D07_H13
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(- )phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions for four days. Root tips were excised and snap frozen (Ross and Gustafson) and total RNA was prepared at University of Missouri, Columbia. Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK(-)phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
attcggcacgagtggacctgggccggcggcatggggtggccaaggtggtg
ggcacggtggtgagcatcggcggcgccacggtgatcacgctctacaaggg
cctgccgctcttcagccacaacctgcacgtgcaggccgtgctctcctgga
cctccggcagccccatcttcaactggaccctcggctgcgtcttcatcctc
ggccactgcctctcctggtcgggatggatggtgctccaggtgcccgtgct
caagcggtacccggcgaggctgtcggtcatctcgctcacctgcgtcttcg
ggctgctccagttcctcgtcatcgccgccttcaccgaggaggacatgagc
cggtggaaggtgcagtccgtcggggagctcttcaccatcctctacgccgg
gctggtggcctccggcgtggcgttcgccctgcagatctggtgcatcgacc
gcggcggcccgctcttcaccgccgtgttccagcccgtgcagaccgtcgcc
gtcgccgtcatggccgccatcatcctcggagaccagctctacaccggagg
gatcatcggcgcggtgctgatcgtcatcgggctctacttcgtgctgtggg
gcaagagcgaggagaagaagacgaggagccaagatcccgagatggcgagg
cacctgctgggggaagacggcggcgaggctgacaaagatcagcaagtgag
caccgctgtgctggcgtgaaagctgctagctgctggatat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.20971.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||